mutation-specific antibodies against braf v600e ve1 clone  (Spring Bioscience)

Journal: Annals of Dermatology

Article Title: Sensitivity and Usefulness of VE1 Immunohistochemical Staining in Acral Melanomas with BRAF Mutation

doi: 10.5021/ad.2018.30.5.556

Figure Lengend Snippet: Nineteen cases with VE1 immunostain grades according to BRAF mutation

Article Snippet: Antigen retrieval was performed with Tris/EDTA buffer solution (pH 9) (Dako, Carpinteria, CA, USA), and primary antibody incubation was performed for 1 hour and 30 minutes at room temperature using the BRAF V600E mutation-specific antibody VE1 (Spring Biosciences, Pleasanton, CA, USA) diluted at 1:100.

Techniques: Mutagenesis, Staining

Journal: Annals of Dermatology

Article Title: Sensitivity and Usefulness of VE1 Immunohistochemical Staining in Acral Melanomas with BRAF Mutation

doi: 10.5021/ad.2018.30.5.556

Figure Lengend Snippet: Clinicopathological features of acral melanomas with BRAF V600E mutation. Acral melanomas (A) on the toe, (B) composed of pagetoid scatter and nests of epithelioid cells (H&E, ×100), and (C) on the finger, (D) showing heavily pigmented epithelioid cells (H&E, ×100).

Article Snippet: Antigen retrieval was performed with Tris/EDTA buffer solution (pH 9) (Dako, Carpinteria, CA, USA), and primary antibody incubation was performed for 1 hour and 30 minutes at room temperature using the BRAF V600E mutation-specific antibody VE1 (Spring Biosciences, Pleasanton, CA, USA) diluted at 1:100.

Techniques: Mutagenesis

Journal: Annals of Dermatology

Article Title: Sensitivity and Usefulness of VE1 Immunohistochemical Staining in Acral Melanomas with BRAF Mutation

doi: 10.5021/ad.2018.30.5.556

Figure Lengend Snippet: VE1 immunohistochemical staining with acral melanomas with BRAF mutation. (A) Negative for VE1 immunostain in a case with BRAF wild-type (Grade 0, ×100). (B) Weak positive staining case with BRAF V600E mutation (Grade 1+, ×100). (C) Moderate positive staining case with BRAF V600E mutation (Grade 2+, ×100). (D) Strong positive staining case with BRAF V600E mutation (Grade 3+, ×100).

Article Snippet: Antigen retrieval was performed with Tris/EDTA buffer solution (pH 9) (Dako, Carpinteria, CA, USA), and primary antibody incubation was performed for 1 hour and 30 minutes at room temperature using the BRAF V600E mutation-specific antibody VE1 (Spring Biosciences, Pleasanton, CA, USA) diluted at 1:100.

Techniques: Immunohistochemical staining, Staining, Mutagenesis

Journal: Annals of Dermatology

Article Title: Sensitivity and Usefulness of VE1 Immunohistochemical Staining in Acral Melanomas with BRAF Mutation

doi: 10.5021/ad.2018.30.5.556

Figure Lengend Snippet: Correlation of 41 acral melanomas with VE1 immunohistochemical stains with BRAF mutation status

Article Snippet: Antigen retrieval was performed with Tris/EDTA buffer solution (pH 9) (Dako, Carpinteria, CA, USA), and primary antibody incubation was performed for 1 hour and 30 minutes at room temperature using the BRAF V600E mutation-specific antibody VE1 (Spring Biosciences, Pleasanton, CA, USA) diluted at 1:100.

Techniques: Immunohistochemical staining, Mutagenesis